Transcriptome Analysis of Vicia villosa in Response to Low Phosphorus Stress at Seedling Stage

Vicia villosa, a high-quality green manure crop, helps to increase the content of soil phosphorus (P) by returning field. Soil P deprivation is severe constraint on crop productivity that triggers low stress response in plants, which controlled various transcriptional regulatory network pathways. Identifying key genes from these networks can help developing P-tolerant crops. In this study, we performed physiological observations and transcriptome sequencing seedlings two villosa varieties, Xu Shao 3 Soviet under starvation conditions. The results showed main root length, plant height, shoot dry weight, acid phosphatase activity, inorganic were significantly higher than those Based data analysis, 183 (shoot) + 144 (root) differential (DEGs) between varieties identified; 79 upregulated, 69 65 downregulated. KEGG analysis found DEGs shoots enriched photosynthesis pathways, such as vitamin B6 riboflavin metabolism. Meanwhile, roots signal transduction, fatty degradation, citric cycle, pentose, glucuronic conversion, etc. GO enrichment suggested biological processes, including cell stress, intracellular ion homeostasis, etc., molecular functions, phosphate ester hydrolase, phosphatase, Furthermore, associated with tolerance included three phosphatases, phosphoesterase, sulfoquinovosyl diacylglycerol synthase, phosphoenolpyruvate carboxylase, six transporters glycerol-3-phosphate transporters, eight SPX, PHL genes. conclusion, exhibited stronger accumulation ability lesser effect growth conditions, might result photosynthesis, sugar, metabolism differences varieties. Acid transporter, SPX leading difference